»
Antibody
»
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 CEACAM5
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody
| Antigen |
|
| Synonyms |
CEA, CD66e, DKFZp781M2392, Psg30, 1600029H12Rik, CEACAM5, CEACAM |
| Clonality |
Monoclonal (CB30) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Immunohistochemistry (Frozen Sections) (IHC (fro)) (36), Enzyme Immunoassay (EIA) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Western Blotting (WB) (26), Flow Cytometry (FACS) (17), Immunofluorescence (IF) (7), Immunoprecipitation (IP) (6), ELISA (3), ELISA (Detection) (3), Cell-ELISA (cELISA) (2), Immunohistochemistry (IHC) (2), Radioimmunoassay (RIA) (2)
|
| Catalog no. |
ABIN192374 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
260.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 3 to 5 Business days |
|
Alternative name
|
CD66e
|
|
Immunogen
|
Human carcinoembryonic antigen (CEA, CEACAM5)
|
|
Format
|
Purified
|
|
Isotype
|
IgG1
|
|
Clone
|
CB30
|
|
Description
|
The CD66e (CEA, 180-200 kDa) is a member of carcinoembryonic antigens,immunoglobulin supergene family and consists of a single N domain (structural homology tothe immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and Bdomains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capableof both homophilic and heterophilic adhesion.Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells.CD66e is found in serum and it is clinically used as a tumor marker for early detection ofdisease due to its expression in adenocarcinomas - potential target of tumor imaging anddrug targeting.
|
|
Specificity
|
The antibody CB30 recognizes CD66e (CEA, 180-200 kDa), a cell surface boundcarcinoembryonic antigen mainly expressed on epithelial cells.
|
|
Application Notes
|
Immunohistochemistry (paraffin sections) - Staining technique: Standard ABC technique (DAB+)Recommended dilution: 10 µg/ml (1: 100) Positive tissue: Adenocarcinoma of colon. Pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature
|
|
Concentration
|
1 mg/ml
|
|
Purity
|
> 95% (by SDS-PAGE)
|
|
Purification
|
Purified from hybridoma culture supernatant by protein-A affinity chromatography.
|
|
Buffer
|
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
|
|
Storage
|
Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
|
|
Research Area
|
CD Antigens, Surface Receptors of Immune Cells
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5)", type "Antibodies"
|
Hosts
|
Mouse (67), Rabbit (13), Goat (1)
|
|
Reactivities
|
Human (77), Mouse (Murine) (1)
|
|
Applications
|
Immunohistochemistry (Frozen Sections) (IHC (fro)) (36), Enzyme Immunoassay (EIA) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Western Blotting (WB) (26), Flow Cytometry (FACS) (17), Immunofluorescence (IF) (7), Immunoprecipitation (IP) (6), ELISA (3), ELISA (Detection) (3), Cell-ELISA (cELISA) (2), Immunohistochemistry (IHC) (2), Radioimmunoassay (RIA) (2)
|
|
Conjugates
|
FITC (3), HRP (1)
|
|
Epitopes
|
Group I (4), Center (1), Group IV (1), Group IVa or II (1)
|
