Stromal Interaction Molecule 1 (STIM1) antibody

Antigen: Stromal Interaction Molecule 1 (STIM1)

Synonyms: GOK, D11S4896E, SIM, stim1, MGC76045, STIM1, MGC154748, si:ch211-112l17.2, Stim1

Clonality: Monoclonal (CDN3H4)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN192394

Additional Information

Alternative name: STIM1

Immunogen: Synthetized peptide (C-terminal cytoplasmic part of STIM1).

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: CDN3H4

Description: STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calciumdepletion within the endoplasmic reticulum and transduces the signal to Orai1, thepresumptive CRAC channel at the plasma membrane. Following decrease of luminalcalcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry ofextracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1interaction has not been elucidated yet. Many questions also remain to be solved aroundSTIM1 functional distribution. It turns out that STIM1 associates with growing ends ofmicrotubules and is involved in endoplasmic reticulum tubule extension.

Specificity: The antibody CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential andconserved regulator of store-operated Ca2+ channel function.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: RBL rat basophilic leukemia cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing conditions. Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Immunocytochemistry - Staining technique: methanol-aceton fixationPositive control: HeLa human cervix carcinoma cell line

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4, methanol-aceton fixation, detection by Goat anti-mouse IgG1 Alexa Fluor® 598, red). Nuclei were stained with DAPI (blue).

Publications

Publications: Ng, Ramduny, Airey et al.: "Orai1 interacts with STIM1 and mediates capacitative Ca2+ entry in mouse pulmonary arterial smooth muscle cells." in: American journal of physiology. Cell physiology, Vol. 299, Issue 5, pp. C1079-90, 2010 (PubMed).