CD300a Molecule (CD300A) antibody

Antigen: CD300a Molecule (CD300A)

Synonyms: IRC1, IRC2, IRp60, IGSF12, CMRF35H, CMRF-35H, CMRF35H9, CMRF-35-H9, Clm8, LMIR1, MMAC8, Pigr4, MAIR-I, mcpir1, MAIR-Ia, B230315M08Rik, CD300L-B1, CD300A, clm8

Clonality: Monoclonal (MEM-260)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB), Radioimmunoassay (RIA)

Catalog no.: ABIN192408

Additional Information

Alternative name: CD300a

Immunogen: HPB human acute lymphoid leukemia cell line

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-260

Description: CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulinsuperfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs)that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressedon many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Itstriggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well assupresses mast cell degranulation or NK cell cytotoxic activity.

Specificity: The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembraneglycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mastcells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

Application Details

Application Notes: Flow CytometryWestern BlottingPositive material: HPB cell lineNegative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.