CD300a Molecule (CD300A) antibody (PE)

Antigen: CD300a Molecule (CD300A)

Synonyms: IRC1, IRC2, IRp60, IGSF12, CMRF35H, CMRF-35H, CMRF35H9, CMRF-35-H9, Clm8, LMIR1, MMAC8, Pigr4, MAIR-I, mcpir1, MAIR-Ia, B230315M08Rik, CD300L-B1, CD300A, clm8

Clonality: Monoclonal (MEM-260)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN192409

Additional Information

Alternative name: CD300a

Immunogen: HPB human acute lymphoid leukemia cell line

Isotype: IgG1

Clone: MEM-260

Description: CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulinsuperfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs)that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressedon many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Itstriggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well assupresses mast cell degranulation or NK cell cytotoxic activity.

Specificity: The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembraneglycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mastcells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Restrictions: For Research Use only

Images

Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.