Signal Transducer CD24 (CD24) antibody (FITC)

Antigen: Signal Transducer CD24 (CD24)

Synonyms: CD24A, FLJ22950, FLJ43543, MGC75043

Clonality: Monoclonal (SN3)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN192413

Additional Information

Alternative name: CD24

Immunogen: Glycoproteins purified from human NALM-1 cell line.

Isotype: IgG1

Clone: SN3

Description: CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-likeGPI-anchored extracellular membrane glycoprotein expressed on several cell types,including B cells. When B cells are activated and induced to further maturation, however,CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, therebyregulating the activity of integrins and other proteins such as the chemokine receptorCXCR4, it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cellprecursors but not in mature resting B cells, where it instead inhibits their ability to proliferatein response to activation. CD24 expression is associated with invasiveness and poorerprognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

Specificity: The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen.CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cellmalignancies. It is not expressed on human thymocytes.HLDA IV, WS Code B 136HLDA V, WS Code B CD24.7

Comments:

CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Surface staining of human peripheral blood cells with anti-CD24 (SN3) Alexa Fluor® 700.

Publications

Publications: Qiu, Low, Chang et al.: "Novel measurements of mammary stem cells in human umbilical cord blood as prospective predictors of breast cancer susceptibility in later life." in: Annals of oncology : official journal of the European Society for Medical Oncology / ESMO, 2011 (PubMed).