phosphodiesterase 11A (PDE11A) antibody

Antigen: phosphodiesterase 11A (PDE11A)

Synonyms: PPNAD2, FLJ23693, MGC133355, MGC133356, Gm350, PDE11A1, 6330414F14Rik, A630086N24Rik, PDE11A, si:ch211-222f23.5, pde11a, wu:ft39g12, Pde11a

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN195735

Additional Information

Alternative name: PDE11A

Gene ID: 50940, 241489 (mouse), 140928 (rat)

Immunogen: C-NSFKESMEKSSYSD

Format: Affinity-purified

Description: All names and symbols: PDE11A, phosphodiesterase 11A , FLJ23693, MGC133355, MGC133356, PPNAD2 , cAMP and cGMP cyclic nucleotide phosphodiesterase 11A, cyclic nucleotide phosphodiesterase 11A, dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A. Official Symbol: PDE11A. RefSeq number(s): NP_058649.3 , NP_001070665.1, NP_001070826.1, NP_001070664.1

Specificity: This antibody is expected to recognise all four reported isoforms (NP_058649.3 , NP_001070665.1, NP_001070826.1, NP_001070664.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx 60-65kDa band observed in Human Skeletal Muscle and Human Prostate lysates (calculated MW of 65.7kDa according to NP_001070826.1). Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Enzymes, Metabolism

Restrictions: For Research Use only

Images

ABIN195735 (1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Horvath, Boikos, Giatzakis et al.: "A genome-wide scan identifies mutations in the gene encoding phosphodiesterase 11A4 (PDE11A) in individuals with adrenocortical hyperplasia." in: Nature genetics, Vol. 38, Issue 7, pp. 794-800, 2006 (PubMed).