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Details for Product No. ABIN197555

Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (Thr451) antibody

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Antigen
Synonyms 2310047A08Rik, 4732414G15Rik, AI467567, AI747578, Pkr, Prkr, Tik, EIF2AK1, PKR, PRKR, pkr, EIF2AK2, DKFZp469K1934
Epitope
»Alternatives Thr451
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 3 references available
Catalog no. ABIN197555
Quantity 50 µg
Price
242.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human PKR around the phosphorylation site of threonine 451 (K-G-Tp-L-R).
Specificity This antibody detects endogenous levels of total PKR protein.
Purification Immunoaffinity Chromatography using epitope-specific immunogen.
Alternative Name EIF2AK2 / PKR
Background PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.
Alternate names: Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced double- stranded RNA-activated protein kinase, PRKR, Protein kinase RNA-activated, eIF-2A protein kinase 2, p68 kinase
Gene ID 5610
NCBI Accession NP_001129123.1
UniProt P19525
Application Notes Immunofluorescence: 1/100~1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Background publications van Huizen, Martindale, Gorospe et al.: "P58IPK, a novel endoplasmic reticulum stress-inducible protein and potential negative regulator of eIF2alpha signaling." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15558-64, 2003 (PubMed).

Ruf, Lackey, Warudkar et al.: "Protection from interferon-induced apoptosis by Epstein-Barr virus small RNAs is not mediated by inhibition of PKR." in: Journal of virology, Vol. 79, Issue 23, pp. 14562-9, 2005 (PubMed).

Hebner, Wilson, Rader et al.: "Human papillomaviruses target the double-stranded RNA protein kinase pathway." in: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3183-93, 2006 (PubMed).

Alternatives for antigen "Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2)", type "Antibodies"
Hosts (203), (9)
Reactivities (207), (63), (32)
Applications (131), (82), (72), (59), (40), (31), (13), (8), (3), (2), (1), (1)
Conjugates (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Epitopes (49), (47), (19), (13), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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