EIF2AK2 antibody (AA 224-502)

Catalog No. ABIN7431893

The Rabbit Polyclonal anti-EIF2AK2 antibody is suitable to detect EIF2AK2 in samples from Human. It has been validated for WB, IHC, IP, ICC and FACS.

Quick Overview for EIF2AK2 antibody (AA 224-502) (ABIN7431893)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-EIF2AK2 Antibody

Binding Specificity: AA 224-502

Purpose: Polyclonal Antibody to Protein Kinase R (PKR)

Specificity: The antibody is a rabbit polyclonal antibody raised against PKR. It has been selected for its ability to recognize PKR in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Kinase R (PKR) corresdonding to Ser224~Ile502 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-5 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: Protein Kinase R

Background: PRKR, PRK-R, EIF2AK2, EIF2AK1, Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, P1/eIF-2A kinase

UniProt: P19525

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C