EIF2AK2 antibody (AA 11-42)

Catalog No. ABIN3030884

The Rabbit Polyclonal anti-EIF2AK2 antibody is suitable to detect EIF2AK2 in samples from Human and Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for EIF2AK2 antibody (AA 11-42) (ABIN3030884)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-EIF2AK2 Antibody

Binding Specificity: AA 11-42

Purpose: PKR Antibody

Purification: Purified

Immunogen: A portion of amino acids 11-42 from the human protein was used as the immunogen for this PKR antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PKR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: PKR

Background: Interferon-induced, double-stranded RNA-activated protein kinase (PRKR/PKR) is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. PRKR, which is involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, activates IKK and NFKB, thereby suppressing apoptosis. Apoptosis induced by live pathogenic gram-positive and gram-negative bacteria requirs both TLR4 and PKR, possibly representing a major mechanism for pathogenic bacteria that use specific virulence factors to avoid detection and destruction by the innate immune system.

UniProt: P19525

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C