TJP3 antibody (Middle Region)

Catalog No. ABIN199820

The Rabbit Polyclonal anti-TJP3 antibody is suitable to detect TJP3 in samples from Human and Dog. It has been validated for WB, IP, ELISA and IF.

Quick Overview for TJP3 antibody (Middle Region) (ABIN199820)

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Product Details anti-TJP3 Antibody

Binding Specificity: Middle Region

Specificity: Recognizes human ZO-3 protein with a molecular weight of ~130kD. Cross-reactivity with other Zonula Occluden family members has not been observed. Reactivity is confirmed with human T84, HeLa, and canine MDCK cell lysates.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the mid region of the human ZO-3 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (0.5 - 2 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 0.5-2 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL. Immunoprecipitation: 2-3 μg.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for TJP3

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Alternative Name: TJP3 / ZO3

Background: Name/Gene ID: TJP3
Family: Occludin

Synonyms: TJP3, Tight junction protein 3, ZO-3, Zona occludens protein 3, Zonula occludens protein 3, Tight junction protein ZO-3, ZO3

Gene ID: 27134

UniProt: O95049