Laminin antibody

Catalog No. ABIN201210

The Rabbit Polyclonal anti-Laminin antibody (ABIN201210) specifically detects Laminin in WB, IHC, IP and ELISA. The antibody is reactive with Human samples.

Quick Overview for Laminin antibody (ABIN201210)

Target: Laminin (LN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA

Product Details anti-Laminin Antibody

Specificity: Recognizes most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross-reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible. Typically less than 1 % cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.

Purification: Immunoaffinity purified

Immunogen: Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:4000), IHC (1:25 - 1:100), IP, WB (1:2500 - 1:5000)

Usage: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation. Western Blot: 1:2500-1:5000. ELISA: 1:2000-1:4000. Immunohistochemistry: 1:25-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 0.01 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Laminin

Target: Laminin (LN)

Alternative Name: Laminin

UniProt: P25391