F4/80 antibody
Quick Overview for F4/80 antibody (ABIN204198)
Target
See all F4/80 (EMR1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- Recognizes a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Cross-reacts with human heart macrophages.
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Purification
- Purified
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Immunogen
- Mouse F4/80.
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Isotype
- IgG2a
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Application Notes
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Approved: Flo (1:25), IHC, IHC-Fr, IHC-P, IP, WB (1:25)
Usage: Suitable for use in Western Blot, Flow Cytometry Immunoprecipitation and Immunohistochemistry. Immunohistochemistry (frozen and paraffin sections): 1:25, F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections. Flow Cytometry: 1:25. Western Blot: 1:25. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, 0.1 % BSA, 0.02 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C,-20 °C
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Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
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Alternative Name
- ADGRE1 / EMR1
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Background
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Name/Gene ID: ADGRE1
Subfamily: Orphan-B
Family: GPCR
Synonyms: ADGRE1, EMR1 hormone receptor, F4/80, EGF-like module receptor 1, TM7LN3, EMR1 -
Gene ID
- 2015
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UniProt
- Q14246
Target
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