WNT8B antibody

Catalog No. ABIN204938

The Rat Monoclonal anti-WNT8B antibody is suitable to detect WNT8B in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for WNT8B antibody (ABIN204938)

Target: WNT8B (Wingless-Type MMTV Integration Site Family, Member 8B (WNT8B))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This WNT8B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-WNT8B Antibody

Specificity: Selected for its ability to detect rmWnt-8b in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rhWnt-2, -7a, -7b, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, or -9b.

Purification: Protein G purified

Immunogen: E. coli-derived rmWnt-8b.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, WB

Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit for rmWnt-8b is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. This antibody was used at 5 μg/mL to detect Wnt-8b in cell extracts. Western Blot buffers are listed below. Wash Buffer Blocking Buffer Blotting Buffer 25 mM Tris,  pH 7.4 5 % nonfat dry milk 2 % nonfat dry milk. 0.5 M NaCl in Wash Buffer in Wash Buffer 0.05 % Tween 20. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human or mouse Wnt-8b. The detection limit for rmWnt-8b is approximately 5 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for WNT8B

Target: WNT8B (Wingless-Type MMTV Integration Site Family, Member 8B (WNT8B))

Alternative Name: WNT8B / Wnt 8b

Background: Name/Gene ID: WNT8B
Family: WNT

Synonyms: WNT8B, Protein Wnt-8b, WNT 8b

Gene ID: 7479

UniProt: Q93098

Pathways: WNT Signaling