Integrin beta 3 antibody (FITC)

Catalog No. ABIN206762

Product Details anti-Integrin beta 3 Antibody (FITC)

Target: Integrin beta 3 (ITGB3)

Reactivity: Mouse, Rat

Host: Hamster

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: Recognizes the murine integrin beta 3 subunit (CD61), a 90kD a type I membrane protein, which is expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptors for a variety of ligands including fibrinogen, fibronectin, von Willebrand's factor (vWF), vitronectin and thrombospondin. Clone HM beta 3-1 is reported to partially inhibit the binding of CD61 to fibronectin. XXX recommend the use of MCA2299EL for this purpose.

Purification: Protein G purified

Immunogen: Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4.

Isotype: IgG

Application Details

Application Notes: Approved: Flo (1:1 - 1:10), IP

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: ITGB3 / Integrin Beta 3 / CD61 (ITGB3 Products)

Background: Name/Gene ID: ITGB3
Family: Integrin

Synonyms: ITGB3, BDPLT2, CD61 antigen, GT, gp3A, CD61, GPIIIa, Integrin beta-3

Gene ID: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex