Band 3/AE1 antibody (AA 184-233)

Catalog No. ABIN6736430

The Rabbit Polyclonal anti-Band 3/AE1 antibody is suitable to detect Band 3/AE1 in samples from Human and Rabbit. It has been validated for WB, IHC and IHC (p).

Quick Overview for Band 3/AE1 antibody (AA 184-233) (ABIN6736430)

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Band 3/AE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Band 3/AE1 Antibody

Binding Specificity: AA 184-233

Specificity: Human SLC4A1 / Band 3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rabbit (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa184-233 of human CHAMP1 (P02730, NP_000333). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Orangutan, Bat (92%), Marmoset (91%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Antibody has been tested in a peptide-based ELISA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Band 3/AE1

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Alternative Name: SLC4A1 / Band 3 / AE1

Background: Name/Gene ID: SLC4A1
Subfamily: Anion exchanger
Family: Transporter

Synonyms: SLC4A1, Anion exchanger 1, Anion exchanger-1, AE1, Anion exchange protein 1, Band 3, Band 3 anion transport protein, BND3, CD233, DI, EPB3, AE 1, Froese blood group, FR, RTA1A, SW, Swann blood group, WD1, WR, Wright blood group, WD, CD233 antigen, EMPB3, Waldner blood group

Gene ID: 6521

NCBI Accession: NP_000333

UniProt: P02730