MYL9 antibody (AA 12-27)

Catalog No. ABIN214356

The Rabbit Polyclonal anti-MYL9 antibody (ABIN214356) specifically detects MYL9 in WB, IHC, ELISA, IP and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MYL9 antibody (AA 12-27) (ABIN214356)

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MYL9 Antibody

Binding Specificity: AA 12-27

Specificity: Amino acids 12-27 of human myosin light chain protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human MYL9.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 μg/mL), IP, WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MYL9

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Alternative Name: MLC2 / MYL9

Background: Name/Gene ID: MYL9
Family: Protein Kinase Non Catalytic

Synonyms: MYL9, 20 kDa myosin light chain, LC20, MLC-2C, MLC2, Myosin RLC, MRLC1, MYRL2

Gene ID: 10398

UniProt: P24844