SAE1 antibody

Catalog No. ABIN214379

The Rabbit Polyclonal anti-SAE1 antibody is suitable to detect SAE1 in samples from Human. It has been validated for WB, ELISA, IF, IHC and IHC (p).

Quick Overview for SAE1 antibody (ABIN214379)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SAE1 Antibody

Specificity: Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST.

Purification: Protein A purified

Immunogen: Recombinant human SAE1.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2 with 0.01 % (w/v) Sodium Azide

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1

Background: Name/Gene ID: SAE1

Synonyms: SAE1, Activator of SUMO1, AOS1, UBLE1A, HSPC140, SUA1

Gene ID: 10055

UniProt: Q9UBE0