Thyroglobulin antibody

Catalog No. ABIN214384

This Mouse Monoclonal antibody specifically detects Thyroglobulin in IHC, WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for Thyroglobulin antibody (ABIN214384)

Target: Thyroglobulin (TG)

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2H11

Product Details anti-Thyroglobulin Antibody

Specificity: Human thyroglobulin protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.

Purification: Protein A purified

Immunogen: Human thyroglobulin protein.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr (1:50 - 1:200), IHC-P (5 μg/mL), WB (1:400)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.5 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: TG / Thyroglobulin

Background: Name/Gene ID: TG

Synonyms: TG, AITD3, TDH3, Thyroglobulin, TGN

Gene ID: 7038

Pathways: Thyroid Hormone Synthesis