PDCD4 antibody (pSer457)

Catalog No. ABIN214444

This Rabbit Polyclonal antibody specifically detects PDCD4 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Xenopus laevis.

Quick Overview for PDCD4 antibody (pSer457) (ABIN214444)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDCD4 Antibody

Binding Specificity: pSer457

Specificity: Amino acids 447-465 of human Pdcd4 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human PDCD4.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:1500 - 1:7000), IHC, IHC-P (1.25 - 2.5 μg/mL), WB (1:200 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1.25-2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4

Background: Name/Gene ID: PDCD4
Family: Apoptosis

Synonyms: PDCD4, H731, Nuclear antigen H731-like, Nuclear antigen H731, Protein 197/15a

Gene ID: 27250

UniProt: Q53EL6