CHRNA3 antibody (AA 26-75)

Catalog No. ABIN6736366

The Rabbit Polyclonal anti-CHRNA3 antibody is suitable to detect CHRNA3 in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Horse and Monkey. It has been validated for WB, IHC and IHC (p).

Quick Overview for CHRNA3 antibody (AA 26-75) (ABIN6736366)

Target: CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHRNA3 Antibody

Binding Specificity: AA 26-75

Specificity: Human CHRNA3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit (100%) Bovine, Guinea pig (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa26-75 of human CHRNA3 (P32297, NP_000734). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Ferret, Elephant, Dog, Bovine, Rabbit, Horse (100%), Pig, Opossum, Guinea pig (92%), Platypus (90%), Mouse, Rat, Hamster (85%), Turkey (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHRNA3

Target: CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Alternative Name: CHRNA3

Background: Name/Gene ID: CHRNA3
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNA3, AChR-alpha3, Acra-3, LNCR2, NAChR alpha 3, PAOD2, NACHRA3

Gene ID: 1136

NCBI Accession: NP_000734

UniProt: P32297

Pathways: Synaptic Membrane