CHRNB3 antibody (AA 176-225)

Catalog No. ABIN6736369

The Rabbit Polyclonal anti-CHRNB3 antibody (ABIN6736369) specifically detects CHRNB3 in WB, IHC and IHC (p). The antibody is reactive with Human, Rat, Mouse, Dog, Guinea Pig, Cow, Rabbit, Chicken and Monkey samples.

Quick Overview for CHRNB3 antibody (AA 176-225) (ABIN6736369)

Target: CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Cow, Rabbit, Chicken, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHRNB3 Antibody

Binding Specificity: AA 176-225

Specificity: Human CHRNB3

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken (100%) Horse (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa176-225 of human CHRNB3 (Q05901, NP_000740). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Guinea pig, Turkey, Zebra finch, Chicken (100%), Galago, Bat, Horse, Opossum (93%), Panda, Platypus (86%), Xenopus (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHRNB3

Target: CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))

Alternative Name: CHRNB3

Background: Name/Gene ID: CHRNB3
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNB3, AChR-beta3, NAChR beta 3

Gene ID: 1142

NCBI Accession: NP_000740

UniProt: Q05901