NFYA antibody (N-Term)

Catalog No. ABIN218318

The Rabbit Polyclonal anti-NFYA antibody is suitable to detect NFYA in samples from Human. It has been validated for WB, ELISA, IHC, IHC (p) and GS.

Quick Overview for NFYA antibody (N-Term) (ABIN218318)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)

Product Details anti-NFYA Antibody

Binding Specificity: N-Term

Specificity: NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Purification: Sterile filtered

Immunogen:

Type of Immunogen: Native protein

Application Details

Application Notes: Approved: ELISA, GS, IHC, IHC-P (1:400), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:400.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Serum - liquid, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NFYA

Background: Name/Gene ID: NFYA

Synonyms: NFYA, CBF-A, CBF-B, HAP2, HAP2 CCAAT-binding protein, NF-YA

Gene ID: 4800

Pathways: Regulation of Lipid Metabolism by PPARalpha