ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3) antibody

Antigen: ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 3 (ASAP3)

Synonyms: ACAP4, UPLC1, CENTB6, DDEFL1, FLJ20199, Gm140, Ddefl1, MGC102639, 9430088F20Rik, ddefl1, MGC113885, zgc:113885

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233800

Additional Information

Alternative name: UPLC1/ASAP3

Gene ID: BAB85677

Swiss-Prot: Q8TDY4

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant human UPLC1/ASAP3 protein.

Format: Liquid (sterile filtered)

Description: UPLC1 (up-regulated in liver cancer 1), also named DDEFL1 (development and differentiation-enhancing factor-like 1) or ASAP3, is a member of the AZAP family of proteins. These proteins catalyze the hydrolysis of GTP bound to ADP-ribosylation factor (Arf) proteins, thereby causing Arf inactivation. For this reason, the ASAPs are generally called ArfGAPs. The activity of ArfGAPs is dependent on the presence of phosphoinositides and is implicated in cellular processes such as membrane trafficking and remodeling of the actin cytoskeleton. Both ASAP1 and ASAP3 have recently been implicated in the development of human cancers. Abnormal activity of ArfGAPs may lead to the alterations characteristic of malignant cells, such as the high rate of proliferation and invasion. More specifically, ASAP3 has been found to be up-regulated in 80% of the hepatocellular carcinomas examined. Initial biochemical characterization reveals that ASAP3 shows class-specific GAP activity on Arf proteins, preferring Arf5 over Arf1 and Arf6.

Specificity: This protein A purified antibody is directed against human UPLC1/ASAP3 protein. The product was purified from monospecific antiserum by protein A chromatography. A BLAST analysis was used to suggest cross-reactivity with UPLC1/ASAP3 protein from mouse and rat based on approximately 80% homology with the human protein. Reactivity against homologues from other sources is not known.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:1,000 - 1:5,000 WESTERN BLOT 1:1000 - 1:10,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. NoteThis protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 100 kDa in size corresponding to UPLC1/ASAP3 protein by western blotting in the appropriate cell lysate or extract.

Concentration: 1.10 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's protein A purified anti-UPLC1/ASAP3 antibody shows detection of UPLC1/ASAP3 in NIH/3T3 cells over-expressing the protein. Cell extracts (5 ug) were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with anti-UPLC1/ASAP3 at a 1:10,000 dilution. Personal Communication, Vi Luan HA, CCR-NCI, Bethesda, MD.