Transmembrane BAX Inhibitor Motif Containing 1 (TMBIM1) antibody

Antigen: Transmembrane BAX Inhibitor Motif Containing 1 (TMBIM1)

Synonyms: RECS1, MST100, PP1201, MSTP100, C78899, Tmbib1, AA960455, AU024746, KIAA4161, mKIAA4161, 2310061B02Rik, MGC94053, TMBIM1, zgc:103528, recs1, mst100, MGC107973, DKFZp469N0332, Tegt, Cc1-27, Ab1-011, Ac1-149, 5031406P05Rik, TMBIM6, BI-1, TEGT, 16.m00782

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN233805

Additional Information

Alternative name: TMBIM1

Gene ID: NP_071435

Swiss-Prot: Q969X1

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of human TMBIM1 protein.

Format: Liquid (sterile filtered)

Description: TMBIM1 (Transmembrane BAX inhibitor motif-containing protein 1) is a member of the 7 TMS (7 transmembrane domains) family of receptors known to mediate the activation of various transcription factors. TMBIM1 is identified as a novel modulator of NF- kappa B activation. A drosophila homolog of this protein (dNMDA1 with 40% homology to hTMBIM1) is reported to be unregulated during aging and oxidative stress.

Specificity: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human TMBIM1 protein. A BLAST analysis was used to suggest partial cross-reactivity with TMBIM1 from mouse and rat sources based on ~88% homology with the immunizing sequence. Cross-reactivity with TMBIM1 from other sources has not been determined.

Synonyms: RECS1, MST100, PP1201, MSTP100, C78899, Tmbib1, AA960455, AU024746, KIAA4161, mKIAA4161, 2310061B02Rik, MGC94053, TMBIM1, zgc:103528, recs1, mst100, MGC107973, DKFZp469N0332, Tegt, Cc1-27, Ab1-011, Ac1-149, 5031406P05Rik, TMBIM6, BI-1, TEGT, 16.m00782

Application Details

Application Notes: Recommended Dilutions: ELISA 1:10,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note. This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kDa in size corresponding to TMBIM-1 by western blotting in the appropriate cell lysate or extract. To date this antibody has shown the ability to recognize over-expressed TMBIM1 but not endogenous protein.

Concentration: 1.2 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Western blot using antibodies-online's protein A purified anti-TMBIM1 antibody shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-hTMBIM1 (lane 1). No staining is observed in lysates from mock transformed HeLa cells (lane 2). To date this antibody has shown the ability to recognize overexpressed TMBIM1 but not endogenous protein. The membrane was probed with the primary antibody at a 1:1,000 dilution at 4

Publications

Publications: Shukla, Fujita, Xiao et al.: "A shear stress responsive gene product PP1201 protects against Fas-mediated apoptosis by reducing Fas expression on the cell surface." in: Apoptosis : an international journal on programmed cell death, 2010 (PubMed).