CD43 antibody

Catalog No. ABIN234645

This anti-CD43 antibody is a Mouse Monoclonal antibody detecting CD43 in FACS, WB, IHC (p) and IP. Suitable for Human.

Quick Overview for CD43 antibody (ABIN234645)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: MEM-59

Product Details anti-CD43 Antibody

Specificity: Recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes. It is negative on resting B lymphocytes and erythrocytes. MEM-59 activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells.

Characteristics: MAb to CD43, Monoclonal antibody to Human CD43, Ascites

Purification: Protein A Chromatography. Ascites

Purity: > 95 % pure (SDS PAGE)

Immunogen: Human T lymphocytes

Isotype: IgG1

Application Details

Application Notes: Suitable for use in Flow cytometry, Immunoprecipitation, Western blot and Immunohistochemistry (paraffin sections). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 15 mM Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Short term store at 2-8 °C. Long term, aliquot and store at -28 °C.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43

Pathways: Regulation of Leukocyte Mediated Immunity