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CD63 gp 53 antibody (FITC)
| Antigen |
|
| Clonality |
Monoclonal (CLBGran-12) |
|
Host
|
Mouse
|
|
Reactivity
|
Human
|
|
Conjugate
|
FITC
|
|
Application
|
Immunofluorescence (IF), Flow Cytometry (FACS)
|
| Catalog no. |
ABIN234721 |
| Quantity |
100 Tests (Not applicable) |
| Price |
583.33 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 to 7 Business Days |
|
Immunogen
|
Human cytochrome B enriched cells
|
|
Format
|
Liquid
|
|
Isotype
|
IgG1
|
|
Clone
|
CLBGran-12
|
|
Description
|
MAb to CD63 Monoclonal Antibody to CD63 gp 53 Fluorescein conjugated
|
|
Specificity
|
The molecular weight of the recognized platelet form of CD63 is 40 to 55kDa. CLBGran/12 reacts with most peripheral blood cells including activated platelets, lymphoid, myeloid and endothelial cells, except for red blood cells and resting T cells. The CD63 antigen is present in many tissues and cell line PHA blasts, K562, U937, KG1a, RAJI. Both surface and cytoplasmotic reactions are reported. CLB Gran/12 has been assigned to the CD63 cluster of differentiation at the IVth International Workshop on Human Leucocyte Differentiation Antigens.
|
|
Application Notes
|
Detection of activated platelets, neutrophils and basophils. Fluorescence microscopy or flow cytometry: 20ul/106 platelets/test or 20ul/5 x 105 cells/test Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
|
|
Concentration
|
Not applicable
|
|
Purification
|
Ion exchange or affinity chromatography FITC:protein molar ratio is 3:10. Source: Ascites
|
|
Buffer
|
0.01M Sodium phosphate, 0.145M Sodium chloride, pH 7.2 containing 2mg/ml BSA. Preservative: 0.1% Sodium azide
|
|
Storage
|
Store (in the dark) at 2-8°C. DO NOT FREEZE.
|
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Restrictions
|
For Research Use only
|
