D-Dimer antibody

Antigen: D-Dimer

Clonality: Monoclonal (DD1)

Host: Mouse

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN234961

Additional Information

Alternative name: Human D-dimer (DD)

Immunogen: Homogenized fibrin clot

Format: Purified, Liquid

Isotype: IgG2a  (Matching secondary antibodies)

Clone: DD1

Description: MAb to D-Dimer Monoclonal Antibody to Human D-dimer (DD)

Specificity: Recognizes Human D-dimer, a specific degradation product of cross-linked fibrin, and high molecular weight fibrin degradation products. No cross-reaction with D-monomer.

Application Details

Application Notes: Suitable for use in ELISA and Western blot. For sandwich immunoassay, plasma samples should be diluted with 10mM Tris/HCl buffer, pH 7.5 containing 1M NaCl and 0.1% Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples should be 0.5M or more. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Recommended pairs for sandwich ELISA: Capture Detection N86340M N86436M N86340M N86043M N86340M N86500M

Concentration: 3.7mg/ml (Sigma protein assay kit)

Purification: >90% pure (SDS-PAGE). Protein A Sepharose chromatography

Buffer: PBS, pH 7.4. Preservative: 0.1% Sodium azide

Storage: Store at 2-8°C.

Restrictions: For Research Use only

Images

D-dimer, D-monomer and fibrinogen immunodetection by MAb DD1 in Western blotting. Lane 1: D-dimer. Lane 2: D-monomer. Lane 3: fibrinogen. Lane 4: D-dimer treated with mercaptoethanol.