Influenza A Nucleoprotein (NP) antibody

Antigen: Influenza A Nucleoprotein (NP)

Clonality: Monoclonal (B248M)

Host: Mouse

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN235631

Additional Information

Immunogen: Purified Influenza virus type A (H1N1)

Format: Purified, Liquid

Isotype: IgG2b  (Matching secondary antibodies)

Clone: B248M

Description: MAb to Influenza A (NP) Monoclonal Antibody to Influenza A Nucleoprotein (NP)

Specificity: Reacts with Influenza Virus type A nucleoprotein. Does not cross-react with Influenza Virus type B nucleoprotein.

Application Details

Application Notes: Suitable for use in ELISA and Western blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Recommended pair for sandwich immunoassay: Capture Detection C01321M C01323M Calibration curve for Influenza A NP immunodetection in sandwich fluoroimmunoassay. Capture: C01321M - 1ug/well Detection (Eu-labeled): C01323M - 0.2ug/well Incubation time 45 minutes H1N1 - Influenza A/New Caledonia/20/99 H3N2 - Influenza A/Shangdong/9/93 Influenza B ? mixture of Influenza B viruses (strains B/Qingdao/102/91, B/Tokio/53/99, B/Victoria/504/00)

Concentration: 4.7mg/ml (Sigma protein assay kit)

Purification: >90% pure (SDS-PAGE). Protein A chromatography. Source: Ascites

Buffer: PBS, pH 7.4. Preservative: 0.1% Sodium azide

Storage: Store at 2-8°C.

Restrictions: For Research Use only

Images

Calibration curve for Influenza A NP immunodetection in sandwich fluoroimmunoassay. Capture: C01321M - 1ug/well. Detection (Eu-labeled): C01323M - 0.2ug/well. Incubation time 45 minutesH1N1 - Influenza A/New Caledonia/20/99. H3N2 - Influenza A/Shangdong/9/93 Influenza B