Helicobacter Pylori (H. pylori) antibody (FITC)

Antigen: Helicobacter Pylori (H. pylori)

Clonality: Polyclonal

Host: Rabbit

Conjugate: FITC

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN237329

Additional Information

Alternative name: Helicobacter pylori

Immunogen: ATCC strain 43504

Format: Liquid

Description: Rabbit a’ Helicobacter pylori Rabbit Antibody to Helicobacter pylori Fluorescein conjugated

Specificity: Whole cell lysate. Has not been absorbed and may react with related microorganisms

Application Details

Application Notes: Suitable for use in direct IFA, ELISA and immunohistochemistry (formalin fixed/paraffin). Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Catalog K65701R and K65702R). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Concentration: 4?5mg/ml (OD280nm, E0.1% 1.4)

Purification: Protein A chromatography. Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Buffer: 0.01M PBS, pH 7.2 containing 10mg/ml BSA. Preservative: 0.1% Sodium azide

Storage: Short term (up to 6 months) at 2-8°C under subdued light. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

Research Area: Bacteria

Restrictions: For Research Use only

Publications

Publications: Lin, Sabri, Alirezaie et al.: "Development of a nanoparticle-labeled microfluidic immunoassay for detection of pathogenic microorganisms." in: Clinical and diagnostic laboratory immunology, Vol. 12, Issue 3, pp. 418-25, 2005 (PubMed).