Mycobacterium Tuberculosis (CFP10) antibody

Antigen: Mycobacterium Tuberculosis (CFP10)

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB)

Catalog no.: ABIN238347

Additional Information

Characteristics: Synonyms: lhp, mtsA10, Rv3874, MT3988, MTV027.09, ESAT-6-like protein esxB, 10 kDa culture filtrateantigen cfp10, Secreted antigenic protein MTSA-10

Alternative name: Cfp10 / esxB

Gene ID: 886194

Swiss-Prot: P0A566

Immunogen: CFP10 (Rv3874) of Mycobacterium tuberculosis

Description: CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated fromMycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigenof T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and arefrequently recognized by T cells from infected people. The genes encoding these proteinshave been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, thatappear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity.

Specificity: The polyclonal antibody reacts with CFP10 (LHP), a 12,3 kDa antigen encoded by geneRv3874 of Mycobacterium tuberculosis. The antibody recognizes CFP10 of Mycobacterium tuberculosis H37Rv and Mycobacteriumbovis, it reacts also with recombinant antigen produced in Escherichia coli.

Application Details

Application Notes: Suitable for Western Blotting under Reducing conditions: 0.2-1 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only