CD73 (AA 27-252) antibody
| Antigen |
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Epitope
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| Clonality |
Monoclonal (2B6) |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN238367 |
| Quantity |
0.1 ml (1.0 mg/ml) (Variants) |
| Price |
360.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: NT5, NTE, NT5E, 5'-nucleotidase
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Swiss-Prot
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P21589
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Immunogen
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Recombinant Human CD73 (aa 27-252) purified from E. coli
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Cross-Reactivity
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Human
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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2B6
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Description
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CD73 (Ecto-5'-nucleotidase, 5'-NT) is a membrane-bound glycoprotein that functions tohydrolyze extracellular nucleoside monophosphates into bioactive nucleosideintermediates. This enables the uptake of adenosine, inosine, and guanosine into the celland their subsequent reconversion into ATP and GTP in the purine salvage pathway. Theexpression of CD73 on neuronal cells has been described during developmental processesand, on lymphocytes, CD73 serves as a maturation marker, being absent from the surfaceof both immature B and T cells.
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Specificity
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This antibody is specific for NT5E/CD73. Species Reactivity: Tested: Human.
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Application Notes
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ELISA. Western blot: 1/1,000 - 1/2,000. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Purified
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Buffer
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PBS, pH 7.4 with 0.09% Sodium Azide as preservative
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD73", type "Antibodies"
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Hosts
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Rabbit (27), Mouse (9)
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Reactivities
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Human (39), Mouse (Murine) (18), Cow (Bovine) (16), Dog (Canine) (16), Rat (Rattus) (16), Chicken (1), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (21), Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), ELISA (11), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (3), AA 27-252 (1), N-Term (1)
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