IgE antibody
| Antigen |
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| Clonality |
Monoclonal (4H10) |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
ELISA (59), Enzyme Immunoassay (EIA) (27), Western Blotting (WB) (27), Flow Cytometry (FACS) (22), Immunofluorescence (IF) (15), Immunodiffusion (ID) (12), Immunoprecipitation (IP) (12), Immunohistochemistry (IHC) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (4), ELISA (Capture) (3), Radioimmunoassay (RIA) (3), ELISA (Detection) (1), Immunoassay (IA) (1), Indirect ELISA (iELISA) (1)
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| Catalog no. |
ABIN238370 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
245.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Immunoglobulin E
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Immunogen
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Purified human IgE.
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Cross-Reactivity
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Human
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Isotype
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IgG1
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Clone
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4H10
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Description
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Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit ofmast cell effector mechanisms. IgE has the lowest serum concentration of allimmunoglobulins (approximately 0. 5 mg/l) in healthy individuals, but upon allergenchallenge its concentration in blood increases dramatically. Although biological survival offree IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinityreceptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergoclonal expansion.
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Specificity
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The antibody 4H10 reacts with human IgE. It recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies toIgE. Species: Human. Others not tested.
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Application Notes
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Suitable for Flow Cytometry and ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Purified
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Buffer
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TBS with 15 mM sodium azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Immunology, Secondary Antibodies
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Restrictions
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For Research Use only
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Alternatives for antigen "IgE", type "Antibodies"
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Hosts
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Mouse (78), Goat (34), Rabbit (7), Sheep (4), Chicken (3), Rat (2), Human (1)
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Reactivities
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Human (107), Mouse (Murine) (9), Dog (Canine) (8), Rat (Rattus) (5)
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Applications
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ELISA (59), Enzyme Immunoassay (EIA) (27), Western Blotting (WB) (27), Flow Cytometry (FACS) (22), Immunofluorescence (IF) (15), Immunodiffusion (ID) (12), Immunoprecipitation (IP) (12), Immunohistochemistry (IHC) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (4), ELISA (Capture) (3), Radioimmunoassay (RIA) (3), ELISA (Detection) (1), Immunoassay (IA) (1), Indirect ELISA (iELISA) (1)
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Conjugates
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HRP (9), FITC (5), Biotin (4), 488-NHS Ester (2), Alkaline Phosphatase (AP) (2), PE-Dyomics 647 (2), Rhodamine (2), Alexa Fluor 488 (1), PE (1)
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Epitopes
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Fc specific (11), Fc (2)
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