Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

CD163 Molecule (CD163) antibody

Antigen

CD163 Molecule (CD163)
Synonyms CD163, DMBT1, M130, MM130, CD163v2, CD163v3
Clonality Monoclonal (5C6-FAT)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN238373
Quantity 1 mg  (2.0 mg/ml)  (Variants)
Price 1,260.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: M130, Hemoglobin scavenger receptor, Macrophage marker, Scavenger receptorcysteine-rich type 1 protein M130
Alternative name CD163
Swiss-Prot Q86VB7
Immunogen Human CD163. The epitope has not been further characterized.
Cross-Reactivity Human
Isotype IgG1  (Matching secondary antibodies)
Clone 5C6-FAT
Description CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and isupregulated by glucocorticoids and IL-10. The extracellular portion of the receptor isregularly shed and can be found in the circulation. An important function of CD163 seemsto be in the adhesion of monocytes to activated endothelial cells. CD163-positive cellsinclude skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and somethymus macrophages. The antigen is also found abundantly in human term placenta, andregularly in acute and chronic inflammatory lesions.
Specificity Monoclonal antibody 5C6 FAT recognizes a membrane glycoprotein on monocytes andmacrophages which is expressed in intermediate and late inflammatory stages. Antigen distribution: Isolated cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days inculture. Does not react with lymphocytes, granulocytes or platelets. Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffercells, few alveolar macrophages, a major population of macrophages in the placenta,varying degrees of macrophages in inflamed tissues, including tumorous tissue depending

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes ELISA. Immunohistochemistry on Frozen Sections: 0.5 μg/ml (1/4000)Immunohistochemistry on Paraffin Sections: 1 μg/ml (1/2000) (Proteinase K pretreatmentfor antigen retrieval is recommended). Suggested Positive Control: Human placenta. Has been described to work in FACS and Western blots. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 2.0 mg/ml
Purification Purified
Buffer PBS, pH 7.2
Storage Store original vial at -70°C. Aliquots of stock solution: at -70°C. Do not freeze working dilutions. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells
Restrictions For Research Use only