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Antibodies
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anti-clathrin, Heavy Chain (Hc) (CLTC) antibody
clathrin, Heavy Chain (Hc) (CLTC) antibody
| Antigen |
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| Synonyms |
Hc, CHC, CHC17, CLH-17, CLTCL2, KIAA0034, CLTC, Chc |
| Clonality |
Monoclonal (BF-06) |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN238388 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: CLTC, CLH17, CLTCL2, KIAA0034, Membrane Vesicle Marker, Clathrin heavy chain onchromosome 17
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Alternative name
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Clathrin heavy chain
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Gene ID
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1213
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Swiss-Prot
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Q00610
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Cross-Reactivity
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Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
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Isotype
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IgM (Matching secondary antibodies)
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Clone
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BF-06
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Description
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Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results inmembrane invagination. The basic oligomers are composed of three clathrin heavy chain(180 kDa) and three light chain (30 kDa) subunits and the process of polymerization isdynamically regulated by the light chains. Interaction of clathrin with the plasmamembrane is mediated by adaptor proteins (AP1-4) specific for different cellularcompartments. Another proteins, such as endophilin, epsin and amphiphysin are involvedin membrane invagination and clathrin rearrangements. Finally, dynamin functions at thefission stage of clathrin-mediated endocytosis.
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Specificity
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The antibody recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa proteininvolved in receptor-mediated endocytosis. Species: Human, Porcine, Mouse, Rat, Bovine. Others not tested.
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Application Notes
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Flow Cytometry: 2-5 ug/ml. Positive control: human blood leukocytes. Immunoprecipitation. Western Blotting. Immunocytochemistry. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Purified
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Buffer
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Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
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Storage
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Store the antibody at 2 - 8 °C. Do not freeze. Shelf life: one year from despatch.
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Research Area
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Organelles, Transporters
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Restrictions
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For Research Use only
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Alternatives for antigen "clathrin, Heavy Chain (Hc) (CLTC)", type "Antibodies"
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Hosts
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Mouse (7), Goat (1), Rabbit (1)
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Reactivities
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Human (6), Cow (Bovine) (4), Rat (Rattus) (4), Mouse (Murine) (2), Pig (Porcine) (2), Amphibian (1), Hamster (1), Primate (1)
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Applications
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Western Blotting (WB) (8), Enzyme Immunoassay (EIA) (3), Immunofluorescence (IF) (3), Immunoprecipitation (IP) (3), ELISA (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1)
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