Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) antibody

Antigen: Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1)

Synonyms: FOLH1, MGC152047

Clonality: Monoclonal (GCP-05)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN238408

Additional Information

Characteristics: Synonyms: Glutamate carboxypeptidase 2, Folate hydrolase 1, Prostate-specific membrane antigen,FOLH, NAALAD1, PSM, GCP2, NAALAdase, Glutamate carboxypeptidase II, Membraneglutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I,Pteroylpoly-gamma-glutamate carboxypeptidase, Folylpoly-gamma-glutamatecarboxypeptidase

Alternative name: PSMA / FOLH1

Swiss-Prot: Q04609

Immunogen: Amino acids 44-750 of Human PSMA/GCPII

Cross-Reactivity: Human

Isotype: IgG1

Clone: GCP-05

Description: Glutamate Carboxypeptidase II (GCPII), also known as N-acetyl-a-linked acidic dipeptidaseI (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen(PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed invarious tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate,which is released from neurons in a calcium-dependent manner, to N-acetylaspartate andglutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes topathological conditions regarding e. g. Alzheimer's disease, Huntington's disease,epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interestingtherapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-g-glutamate to folate andglutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is presentin a number of tissues at low levels, is overexpressed in neovasculature of most solidtumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normalhuman prostate express more mRNA coding for a cytosolic GCPII form truncated at theN-terminus (PSM') than mRNA for membrane-bound GCPII, and this ratio is reversed uponmalignant transformation.

Specificity: The antibody recognizes extracellular domain of glutamate carboxypeptidase II(NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoproteinexpressed mainly in tumour neovasculatures, nervous system and jejunum, which is animportant prostate tumour marker. Species Reactivity: Tested: Human.

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide aspreservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only