IgG (Light Chain) antibody
| Antigen |
|
|
Epitope
|
H&L (247), F(ab')2 (89), Fc Region (49), Fc specific (32), Fc (31), Chain gamma (2), F(ab')3 (1), F(ab')4 (1), F(ab')5 (1), F(ab')6 (1), F(ab')7 (1), F(ab')8 (1), Light chain (1)
|
| Clonality |
Monoclonal (IVA285-1) |
|
Host
|
|
|
Reactivity
|
Human (188), Guinea Pig (96), Rabbit (96), Cow (Bovine) (80), Cat (Feline) (68), Goat (67), Chicken (55), Dog (Canine) (55), Mouse (Murine) (53), Horse (Equine) (52), Rat (Rattus) (43), Pig (Porcine) (35), Sheep (Ovine) (29), Donkey (27), Golden Syrian Hamster (21), Monkey (16), Hamster (13), Syrian Hamster (12), Armenian Hamster (9), Llama (2), Turkey (2), All Species (1), Alligator (1), Cynomolgus (1), Duck (1), Ferret (1), Mink (1)
|
|
Application
|
 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
ELISA (373), Immunofluorescence (IF) (225), Flow Cytometry (FACS) (220), Western Blotting (WB) (220), Immunohistochemistry (IHC) (179), Immunoprecipitation (IP) (88), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (59), Enzyme Immunoassay (EIA) (44), Immunohistochemistry (Frozen Sections) (IHC (fro)) (38), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (29), Immunodiffusion (ID) (20), Immunocytochemistry (ICC) (16), Immunoassay (IA) (12), Radioimmunoassay (RIA) (4), Dot Blot (Dot) (3), Lateral Flow (LF) (3), ELISA (Capture) (1), ELISA (Detection) (1), Gel Shift (GS) (1), Immunoelectrophoresis (IEP) (1)
|
| Catalog no. |
ABIN238423 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
265.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Characteristics
|
Synonyms: Immunoglobulin G
|
|
Immunogen
|
Fraction of Bovine immunoglobulin.
|
|
Cross-Reactivity
|
Cow (Bovine)
|
|
Isotype
|
IgG1
|
|
Clone
|
IVA285-1
|
|
Specificity
|
The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nadlambda types) consist of a variable region and a constant region and can easily bedifferentiated by the antigenic properties of the constant region. Species: Bovine. Others not tested.
|
|
Application Notes
|
Western Blotting (Reducing conditions): 0,5-1 μg/ml, overnight in 4C. SDS-PAGE (12% separating gel). Positive control and sample preparation: BS (bovine serum), dilution 1: 50 in Laemmlireducing buffer, boiled in water bath for 3 min. Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry (frozen sections). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
|
|
Concentration
|
1.0 mg/ml
|
|
Purification
|
Aff - Purified
|
|
Buffer
|
PBS with 15 mM sodium azide as preservative.
|
|
Storage
|
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "IgG", type "Antibodies"
|
Hosts
|
Goat (448), Rabbit (437), Mouse (72), Donkey (40), Sheep (26), Chicken (14), Rat (3), Human (2), Cynomolgus Monkey (1), Guinea Pig (1)
|
|
Reactivities
|
Human (188), Guinea Pig (96), Rabbit (96), Cow (Bovine) (80), Cat (Feline) (68), Goat (67), Chicken (55), Dog (Canine) (55), Mouse (Murine) (53), Horse (Equine) (52), Rat (Rattus) (43), Pig (Porcine) (35), Sheep (Ovine) (29), Donkey (27), Golden Syrian Hamster (21), Monkey (16), Hamster (13), Syrian Hamster (12), Armenian Hamster (9), Llama (2), Turkey (2), All Species (1), Alligator (1), Cynomolgus (1), Duck (1), Ferret (1), Mink (1)
|
|
Applications
|
ELISA (373), Immunofluorescence (IF) (225), Flow Cytometry (FACS) (220), Western Blotting (WB) (220), Immunohistochemistry (IHC) (179), Immunoprecipitation (IP) (88), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (59), Enzyme Immunoassay (EIA) (44), Immunohistochemistry (Frozen Sections) (IHC (fro)) (38), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (29), Immunodiffusion (ID) (20), Immunocytochemistry (ICC) (16), Immunoassay (IA) (12), Radioimmunoassay (RIA) (4), Dot Blot (Dot) (3), Lateral Flow (LF) (3), ELISA (Capture) (1), ELISA (Detection) (1), Gel Shift (GS) (1), Immunoelectrophoresis (IEP) (1)
|
|
Conjugates
|
HRP (108), FITC (106), Rhodamine (92), Biotin (89), Alkaline Phosphatase (AP) (87), Texas Red (TR) (40), 488-NHS Ester (9), PE (7), DY549 (1), DY649 (1)
|
|
Epitopes
|
H&L (247), F(ab')2 (89), Fc Region (49), Fc specific (32), Fc (31), Chain gamma (2), F(ab')3 (1), F(ab')4 (1), F(ab')5 (1), F(ab')6 (1), F(ab')7 (1), F(ab')8 (1), Light chain (1)
|
