CD63 antibody (PE)

Antigen: CD63

Clonality: Monoclonal (MEM-259)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN238464

Additional Information

Swiss-Prot: P08962

Immunogen: HPB-ALL T cell line

Cross-Reactivity: Human

Isotype: IgG1

Clone: MEM-259

Description: CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T- cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. Alternate Names: LAMP-3, Lysosome-Associated Membrane Protein-3.

Specificity: The antibody MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Application Details

Application Notes: Suitable for Flow Cytometry analysis of human blood cells using 20 m l reagent / 100 m l of whole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.

Storage: Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf Life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only