CD300a Molecule (CD300A) antibody

Antigen: CD300a Molecule (CD300A)

Synonyms: IRC1, IRC2, IRp60, IGSF12, CMRF35H, CMRF-35H, CMRF35H9, CMRF-35-H9, Clm8, LMIR1, MMAC8, Pigr4, MAIR-I, mcpir1, MAIR-Ia, B230315M08Rik, CD300L-B1, CD300A, clm8

Clonality: Monoclonal (MEM-260)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN238465

Additional Information

Characteristics: Synonyms: CLM-8, CMRF35-H, CMRF35-H9, CMRF-35-H9, IRC1/IRC2, IRp60, CMRF35-like molecule 8,Inhibitory receptor protein 60, NK inhibitory receptor, CD300 antigen-like family member A

Alternative name: CD300A / CMRF35H

Swiss-Prot: Q9UGN4

Immunogen: HPB human acute lymphoid leukemia cell line.

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-260

Description: CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulinsuperfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs)that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressedon many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Itstriggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well assupresses mast cell degranulation or NK cell cytotoxic activity.

Specificity: This antibody reacts with CD300a, a 60 kDa leukocyte transmembrane glycoproteinexpressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells anddendritic cells, 25% of circulating T cells and 15% of circulating B cells. Species: Human. Others not tested.

Application Details

Application Notes: Flow Cytometry. Western Blotting (Non-reducing conditions): Positive material: HPB cell lineNegative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only