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Antibodies
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anti-Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D) antibody
Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D) antibody
| Antigen |
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| Synonyms |
SHIP, SHIP1, hp51CN, SIP-145, MGC104855, MGC142140, MGC142142, INPP5D, MGC157261, inpp5d, ship, ship1, MGC68993 |
| Clonality |
Monoclonal (SHIP-02) |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Western Blotting (WB)
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| Catalog no. |
ABIN238500 |
| Quantity |
0.1 mg (1.0 mg/ml) |
| Price |
265.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: SHIP, SHIP1, SHIP-1, p150Ship, SIP-145, Phosphatidylinositol-3, 4, 5-trisphosphate5-phosphatase 1, SH2 domain-containing inositol-5'-phosphatase 1, Inositolpolyphosphate-5-phosphatase of 145 kDa
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Alternative name
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INPP5D
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Gene ID
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3635
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Swiss-Prot
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Q92835
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Immunogen
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Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
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Cross-Reactivity
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Human
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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SHIP-02
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Description
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SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase thatregulates cell responses in lymphocytes and myeloid cells by hydrolyzing the secondmessenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitoryand activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine andgrowth factor receptors, and supresses PI3K-dependent signaling, down-regulates cellmigration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as ascaffold for the recruitment of other proteins to the plasma membrane.
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Specificity
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The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confinedto hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weightsof 110, 125, 130, 135 and 145 kDa. Species: Human.
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Application Notes
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Western Blotting (Reducing conditions)Positive control: RAMOS human cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Aff - Purified
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Buffer
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PBS, pH 7.4 with 15 mM sodium azide as preservative.
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Storage
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Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D)", type "Antibodies"
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Hosts
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Rabbit (40), Mouse (5)
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Reactivities
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Human (43), Mouse (Murine) (27), Rat (Rattus) (25), Cow (Bovine) (2), Pig (Porcine) (2)
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Applications
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Flow Cytometry (FACS) (23), Western Blotting (WB) (20), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), ELISA (8), Enzyme Immunoassay (EIA) (7), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (4), Center (2), N-Term (2), pTyr1021 (1)
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