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Alpha Tubulin / TUBA1B antibody

Antigen

Alpha Tubulin / TUBA1B
Clonality Monoclonal (TU-02)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN238516
Quantity 0.1 mg  (1.0 mg/ml)
Price 265.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: Microtubule Marker, Loading Control, alpha-Tubulin, tubulin alpha
Immunogen microtubule proteins from porcine brain
Cross-Reactivity Human, Mouse (Murine), Pig (Porcine)
Isotype IgM  (Matching secondary antibodies)
Clone TU-02
Description The microtubules are intracellular dynamic polymers made up of evolutionarily conservedpolymorphic alpha/beta-tubulin heterodimers and a large number ofmicrotubule-associated proteins (MAPs). The microtubules consist of 13 protofilamentsand have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highlydynamic plus ends and less dynamic minus ends. Microtubules are required for vitalprocesses in eukaryotic cells including mitosis, meiosis, maintenance of cell shape andintracellular transport. Microtubules are also necessary for movement of cells by means offlagella and cilia. In mammalian tissue culture cells microtubules have their minus endsanchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate)molecule is an essential for tubulin heterodimer to associate with other heterodimers toform microtubule. In vivo, microtubule dynamics vary considerably. Microtubulepolymerization is reversible and a populations of microtubules in cells are on their minusends either growing or shortening - this phenomenon is called dynamic instability ofmicrotubules. On a practical level, microtubules can easily be stabilized by the addition ofnon-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugssuch as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Usinglimited proteolysis by enzymes both tubulin subunits can be divided into N-terminal andC-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists incells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. Indifferent species it is coded by multiple tubulin genes that form tubulin classes (in human6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulinisotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulinisotypes and their posttranslational modifications are responsible for multiple tubulincharge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated inC-terminal structural domain.
Specificity The antibody TU-02 recognizes an epitope on N-terminal structural domain of alpha-tubulinin various species. Species: Human, Porcine, Mouse. Others not tested.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Western Blotting (Reducing conditions): 1 μg/mlPositive control: porcine brain lysate. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Purified
Buffer PBS, pH 7.4 with 15 mM sodium azide as preservative.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Restrictions For Research Use only