Chromosome 14 Open Reading Frame 169 (C14orf169) antibody

Antigen: Chromosome 14 Open Reading Frame 169 (C14orf169)

Synonyms: NO66, MAPJD, FLJ21802, LOC453015

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238538

Additional Information

Alternative name: C14orf169 / NO66

Gene ID: 79697, 71952 (mouse)

Immunogen: C-GAQHSDSKDPR

Format: Affinity-purified

Description: All names and symbols: C14orf169, NO66, chromosome 14 open reading frame 169, FLJ21802, MAPJD. Official Symbol: C14orf169. RefSeq number(s): NP_078920.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in lysates of cell line A431 (calculated MW of 71.1kDa according to NP_078920.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

ABIN238538 (0.3µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Eilbracht, Reichenzeller, Hergt et al.: "NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin." in: Molecular biology of the cell, Vol. 15, Issue 4, pp. 1816-32, 2004 (PubMed).