Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) antibody

Antigen: Insulin-Like Growth Factor Binding Protein 3 (IGFBP3)

Synonyms: IBP3, BP-53, IGFBP-3, IGgfbp3, AI649005, IGF-BP3, MGC156773, IGFBP3, MGC91788, zgc:91788, igfbp3, DKFZp468B159

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238551

Additional Information

Alternative name: IGFBP3

Gene ID: 3486, 16009 (mouse), 24484 (rat)

Immunogen: C-RYKVDYESQSTDTQN

Description: All names and symbols: IGFBP3, insulin-like growth factor binding protein 3, tcag7.703, BP-53, IBP3, IGF-binding protein 3, acid stable subunit of the 140 K IGF complex , binding protein 29, binding protein 53, growth hormone-dependent binding protein. Official Symbol: IGFBP3 . RefSeq number(s): NP_001013416.1, NP_000589.2

Specificity: This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 28kDa band observed in Human Breast cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06µg/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cardiovascular, Atherosclerosis, Angiogenesis, Apoptosis/Necrosis, Hormones, Diabetes, Metabolism, Growth Factors

Restrictions: For Research Use only

Images

ABIN238551 (0.02µg/ml) staining of Human Breast cancer lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Lin, Manson, Lee et al.: "Intakes of calcium and vitamin D and breast cancer risk in women." in: Archives of internal medicine, Vol. 167, Issue 10, pp. 1050-9, 2007 (PubMed).