Potassium Inwardly-Rectifying Channel, Subfamily J, Member 6 (KCNJ6) antibody

Antigen: Potassium Inwardly-Rectifying Channel, Subfamily J, Member 6 (KCNJ6)

Synonyms: BIR1, GIRK2, KATP2, KCNJ7, KIR3.2, hiGIRK2, MGC126596, wv, Kir3.2, weaver, KCNJ6, kcnj6, DKFZp459A0820

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238558

Additional Information

Alternative name: GIRK2 / KCNJ6

Gene ID: 3763, 16522 (mouse), 25743 (rat)

Immunogen: Peptide with sequence C-SSKLNQHAELET, from the C Terminus of the protein sequence according to NP_002231.1.

Format: Affinity-purified

Description: All names and symbols: KCNJ6, GIRK2, potassium inwardly-rectifying channel, subfamily J, member 6, BIR1, KATP2, KCNJ7, KIR3.2, MGC126596, hiGIRK2, G protein-activated inward rectifier potassium channel 2, inward rectifier potassium channel KIR3.2, potassium inwardly-. Official Symbol: KCNJ6 . RefSeq number(s): NP_002231.1

Specificity: Expected from sequence similarity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 48kDa band observed in Human Brain (Hippocampus and Substantia Nigra) lysates (calculated MW of 48.5kDa according to NP_002231.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide.

Concentration: 0.5 mg/ml

Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238558 (1µg/ml) staining of Human Brain (Substantia Nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Lu, Lin, Huang et al.: "XIAP induces NF-kappaB activation via the BIR1/TAB1 interaction and BIR1 dimerization." in: Molecular cell, Vol. 26, Issue 5, pp. 689-702, 2007 (PubMed).