Cell Adhesion Molecule 4 (CADM4) (internal) antibody

Antigen: Cell Adhesion Molecule 4 (CADM4)

Synonyms: NECL4, TSLL2, IGSF4C, Necl-4, synCAM4, CADM4, cadm4, MGC85352, zgc:101704

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238560

Additional Information

Alternative name: CADM4 (internal)

Gene ID: 199731, 260299 (mouse)

Immunogen: C-KDERFQLEEFS

Format: Affinity-purified

Description: All names and symbols: CADM4, cell adhesion molecule 4, IGSF4C, Necl-4, TSLL2, synCAM4, TSLC1-like 2, immunoglobulin superfamily, member 4C, nectin-like 4. Official Symbol: CADM4. RefSeq number(s): NP_660339.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 42.8kDa according to NP_660339.1). The observed molecular weight corresponds to findings with ABIN238617, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

Staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). A) EB08040 (0.1µg/ml) and B) ABIN238617 (0.05µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Spiegel, Adamsky, Eshed et al.: "A central role for Necl4 (SynCAM4) in Schwann cell-axon interaction and myelination." in: Nature neuroscience, Vol. 10, Issue 7, pp. 861-9, 2007 (PubMed).