Early Growth Response 4 (EGR4) antibody

Antigen: Early Growth Response 4 (EGR4)

Synonyms: NGFIC, NGFI-C, PAT133, NGF1-C, pAT133, Egr4l1, EGR4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238575

Additional Information

Alternative name: EGR4

Gene ID: 1961

Immunogen: NCGSQGDYQAAPE

Format: Affinity-purified

Description: All names and symbols: EGR4, early growth response 4, NGFI-C, NGFIC, PAT133. Official Symbol: EGR4. RefSeq number(s): NP_001956.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 50kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 50.8kDa according to NP_001956.2). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN238575 (0.3µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Uvarov, Ludwig, Markkanen et al.: "Upregulation of the neuron-specific K+/Cl- cotransporter expression by transcription factor early growth response 4." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 52, pp. 13463-73, 2006 (PubMed).