Hypocretin (Orexin) Receptor 2 (HCRTR2) antibody

Antigen: Hypocretin (Orexin) Receptor 2 (HCRTR2)

Synonyms: OX, PPOX, NRCLP1, MGC151320, MGC151346, orexin-A, HCRTR2, HCRT, LOC100229747, OX2R, LOC443181, hcrtr

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238589

Additional Information

Alternative name: Orexin Receptor 2

Gene ID: 3062, 25605 (rat)

Immunogen: SGTKLEDSPPCRN

Format: Affinity-purified

Description: All names and symbols: OTTHUMP00000039969, hypocretin (orexin) receptor 2, HCRTR2, OX2R, hypocretin receptor-2, orexin receptor 2. Official Symbol: HCRTR2 . RefSeq number(s): NP_001517.1

Specificity: Human, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 50kDa band observed in human brain (cerebral cortex, hippocampus and substantia nigra) lysates (calculated MW of 50.7kDa according to NP_001517.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Hormones, Receptors

Restrictions: For Research Use only

Images

ABIN238589 (1µg/ml) staining of human brain (hippocampus) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Spinazzi, Rucinski, Neri et al.: "Preproorexin and orexin receptors are expressed in cortisol-secreting adrenocortical adenomas, and orexins stimulate in vitro cortisol secretion and growth of tumor cells." in: The Journal of clinical endocrinology and metabolism, Vol. 90, Issue 6, pp. 3544-9, 2005 (PubMed).