Puromycin Sensitive Aminopeptidase (PSA) antibody

Antigen: Puromycin Sensitive Aminopeptidase (PSA)

Synonyms: PSA, MP100, dPsa, DmelCG1009, CG1009, GB13772

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238593

Additional Information

Alternative name: MP100 / NPEPPS

Gene ID: 9520

Immunogen: C-KRDAESIHQYLLQRK

Format: Affinity-purified

Description: All names and symbols: NPEPPS, MP100, aminopeptidase puromycin sensitive, PSA, metalloproteinase MP100, puromycin-sensitive aminopeptidase. Official Symbol: NPEPPS. RefSeq number(s): NP_006301.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 110kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 98.5kDa according to NP_006301.2). Recommended concentration: 0.2-0.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238593 (0.3µg/ml) staining of Human Brain (Cerebral Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Karsten, Sang, Gehman et al.: "A genomic screen for modifiers of tauopathy identifies puromycin-sensitive aminopeptidase as an inhibitor of tau-induced neurodegeneration." in: Neuron, Vol. 51, Issue 5, pp. 549-60, 2006 (PubMed).