WNT1 Inducible Signaling Pathway Protein 1 (WISP1) antibody

Antigen: WNT1 Inducible Signaling Pathway Protein 1 (WISP1)

Synonyms: CCN4, WISP1c, WISP1i, WISP1tc, WISP-1, WISP1, si:ch211-38f10.2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238610

Additional Information

Alternative name: WISP1

Gene ID: 8840, 22402 (mouse), 65154 (rat)

Immunogen: C-ESYPDFSEIAN

Description: All names and symbols: WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1. Official Symbol: WISP1 . RefSeq number(s): NP_003873.1, NP_543028.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1). Human, Mouse, Rat,

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 45-48kDa band observed in lysates of HEK293 (calculated MW of 40.3kDa according to NP_003873.1). The observed molecular weight corresponds to earlier findings with differen

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Stem Cells, Wnt, Transcription Factors, DNA/RNA

Restrictions: For Research Use only

Images

ABIN238610 (0.1 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Major, Camp, Berndt et al.: "Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling." in: Science (New York, N.Y.), Vol. 316, Issue 5827, pp. 1043-6, 2007 (PubMed).