Cysteine and Glycine-Rich Protein 2 (CSRP2) antibody

Antigen: Cysteine and Glycine-Rich Protein 2 (CSRP2)

Synonyms: CRP2, LMO5, SmLIM, Crp2, SmLim, AW551867, MGC77740, zgc:77740, BETA-CRP, MGC86339, crp2, lmo5, smlim, MGC81964, csrp2, MGC89085, MGC133755, csrp1, CSRP2

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238612

Additional Information

Alternative name: CRP2 / CSRP2

Gene ID: 1466, 13008 (mouse)

Immunogen: C-ERLGIKPESVQP

Format: Affinity-purified

Description: All names and symbols: CRP2, CSRP2, cysteine and glycine-rich protein 2, LMO5, SmLIM, LIM domain only 5, smooth muscle. Official Symbol: CSRP2. RefSeq number(s): NP_001312.1

Specificity: Human, Mouse, Rat, Horse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25,275(8):5504-11, PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238612 (0.1µg/ml) staining of mouse kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CRP2 (RC201565) and probed with ABIN238612 (mock transfection in first lane), tested by Origene.

Publications

Publications: Stearns, Wang, Hu et al.: "Interleukin-10 activation of the interleukin-10E1 pathway and tissue inhibitor of metalloproteinase-1 expression is enhanced by proteasome inhibitors in primary prostate tumor lines." in: Molecular cancer research : MCR, Vol. 1, Issue 9, pp. 631-42, 2003 (PubMed).