Proenkephalin (PENK) antibody

Antigen: Proenkephalin (PENK)

Synonyms: ENK, PPA, Penk1, AI326464, PENK, penk, MGC134476, Enk, Penk2, Penk-rs, MGC93514, zgc:73353, Penk, MGC107924

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238618

Additional Information

Alternative name: Proenkephalin

Gene ID: 5179

Immunogen: C-RSHHQDGSDNEE

Format: Affinity-purified

Description: All names and symbols: proenkephalin, PENK. Official Symbol: PENK. RefSeq number(s): NP_006202.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology. 1998 Apr,139(4):2137-45, PMID: 9529003). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238618 (0.3µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Schiltz, Bremer, Landry et al.: "Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression." in: BMC biology, Vol. 5, pp. 16, 2007 (PubMed).